Inhibition of Antiassociation Activity of Translation Initiation Factor 3 by Paromomycin

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Inhibition of antiassociation activity of translation initiation factor 3 by paromomycin.

The effect of paromomycin on the interaction of ribosomal subunits was studied. Paromomycin inhibited the antiassociation activity of initiation factor 3 (IF3). Furthermore, ribosomal subunits were associated to form 70S ribosomes by paromomycin even in the presence of 1 mM Mg(2+). Paromomycin did not inhibit the binding of IF3 to the 30S ribosomal subunits. On the other hand, IF3 bound to the ...

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Although much is known about the multiple mechanisms which induce apoptosis, comparatively little is understood concerning the execution phase of apoptosis and the mechanism(s) of cell killing. Several reports have demonstrated that cellular translation is shut off during apoptosis; however, details of the mechanism of translation inhibition are lacking. Translation initiation factor 4G (eIF4G)...

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3-Deazaguanine: inhibition of initiation of translation in L1210 cells.

In L1210 cells in culture, 3-deazaguanine (DG), a relatively new purine analog, was found to inhibit DNA and protein synthesis but not total RNA synthesis. The effect of the drug on protein synthesis was therefore further examined. Polyadenylic acid-containing RNA synthesis was not decreased by DG treatment, suggesting that the inhibiton of protein synthesis was a function of an alteration in t...

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3-Deazaguanine: Inhibition of Initiation of Translation in L1210 Cells1

In L1210 cells in culture, 3-deazaguanine (DG), a relatively new purine analog, was found to inhibit DNA and protein synthesis but not total RNA synthesis. The effect of the drug on protein synthesis was therefore further examined. Polyadenylic acid-containing RNA synthesis was not decreased by DG treatment, suggesting that the inhibition of protein synthesis was a function of an alteration in ...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 2007

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.01096-06